Phytochemicals, hypoglycemic and hypolipidemic effects of. Original article rat model of foodinduced nonobesetype. Antihyperglycemic effect was investigated in normal and alloxan induced diabetic rats. In this study, oral administration of zanthoxylum chalybeum at different doses for 4 weeks was evaluated for effects of z. Biochemical and haematological effects of the leaf extract. The rats randomly selected were divided into six 6 groups of five 5 rats per group. Hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats.
The alloxan treated rats with permanent hyperglycemia, which further received i. The extract improve the liver, kidney and pancreas function and reduce lesions associated with diabetic st ate in alloxan induced rats. May 24, 2014 parallel to our result, ghannnam et al. Dec 18, 2007 alloxan has two distinct pathological effects. What is the right dose of alloxan to induce diabetes 1 in albino rats. The extracts of berberis lycium and diabetes mellitus in alloxan monohydrate induced diabetic rats. Treatment of diabetic rats with sodium phthalhydrazide partially reversed diabetogenic pathology in the alloxan induced diabetes. Antidiabetic activity of aqueous extract of leptospermum. Marked degenerations of the islets of langerhans was observed in pancreas of alloxan induced diabetic rats, whereas, histological examination of the pancreas of. Normal and diabetic control rats received saline, whereas rats in other groups received 300 or 600 mgkg body weight of extract or metformin 100 mgkg body weight of metformin for 6 weeks. The mechanisms of alloxan and streptozotocininduced.
Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Effect of ethanolic extract of pterocarpus marsupium root on. Blood glucose level and lipid profile of alloxaninduced. Sensitization to alloxan induced diabetes and pancreatic cell apopto. Antidiabetic effect of hydroalcoholic extract of carthamus tinctorius l.
Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Alloxan induced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16. Studies on the administration of vernonia amygdalina del. The test group was treated with 200 mgkg dosage of moringa oleifera ethanolic leave extract. Hypoglycemic and antioxidant effects of leaf essential oil. Periodontal disease in diabetic patients presents higher severity and prevalence. Hypoglycemic effects of aeso in alloxaninduced diabetic rats. Comparison of streptozotocin and alloxaninduced diabetes in. Antidiabetic effect of ipomoea batatas in normal and alloxan.
Hemoglobin, packed cell volume, platelets, red blood cells, serum glucose, white blood cells, zingiber officinale. Pdf the antidiabetic activity of the hf consisting of three wellknown herbs for diabetes namely, piper betel, butea monosperma and. Viswanathaswamy, et al antihyperglycemic and antihyperlipidemic activity of plectranthus amboinicus the present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of plectranthus amboinicus in normal and alloxan induced diabetic rats. Most studies have shown that alloxan is not toxic to the human betacell, even in very high doses, probably because of differing glucose uptake mechanisms in humans and rodents. Antidiabetic effect of ipomoea batatas in normal and alloxan induced diabetic rats. Significant decrease in body weight also was observed with. The mechanism of alloxan and streptozotocin action in b cells. Vitamin c and vitamin e on wound healing in alloxaninduced diabetic rats a tas1, a karasu1, z yener2, s y. Alloxaninduced diabetes mellitus how is alloxaninduced.
Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. In indias indigenous system of medicine, coriandrum sativum cs, commonly used as a food ingredient, is claimed to be useful for various ailments. Fabaceae leaves and flowers on alloxan induced diabetes in rats. Antidiabetic activity of alcoholic extract of leaves of colocsia. Research article antidiabetic effect of sida cordata in. Antidiabetic effect of sida cordata in alloxan induced diabetic rats. Group 3 diabetic rats that were given leucas lavandulaefolia. The blood sugar and body weight of the rats was recorded at two weeks and four weeks interval, and one week after the withdrawal of the extract. Alloxan definition of alloxan by medical dictionary.
At the end of the treatment period, blood glucose levels, protein, mda, catalase, glutathione and vitamin c concentrations were determined in both the. Diabetes was induced in albino rats by administration of alloxan. Effects of the aqueous extract on groups of alloxan 150mgkg induced diabetic rats was investigated. The experimental design comprises of five 5 groups of four 4 animals each. The mechanisms of alloxan and streptozotocin induced diabetes, diabetologia, 2007, pp. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antidiabetic activity of ethanol extract of colocasia esculenta leaves in alloxan induced diabetic rats. Red kidney beans was investigated for its effects in alloxan induced diabetic rats.
Antidiabetic effect of ipomoea batatas in normal and. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxan induced diabetic male rats. Wheatgrass has many characteristics desirable for health. Sep 30, 2016 in the current investigation, a statistical model for the optimal dose level of alloxan and stz were recognized in order to induce dm in wistar male rats.
Protective effects of zanthoxylum chalybeum in diabetes. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. It has been successfully induced in a variety of animal species. Antidiabetic activity of nerium indicum leaf extract in alloxan. Antidiabetic and hypolipidaemic properties of ginger. The rats were divided into six groups after the induction of alloxan diabetes. Diabetes was induced in overnight fasted rats by intraperitoneal injection. Wound healing activity of ethanolic extract of allium sativum on alloxan induced diabetic rats family liliaceae mohammad. This work evaluated the effects of different doses of the human urine on normal and alloxan induced diabetic rats. The aim of the study was to evaluate the anti diabetic effect of isolated compound from p. Leaf extracts in alloxan induced diabetic rats syed mansoor ahmed, vrushabendra swamy bm, p gopkumar r dhanapal and vm chandrashekara department of pharmacology, m. Antidiabetic activity of compound isolated from physalis.
Alloxan was injected intraperitoneally at a single dose of 150mgkg body weight b. To study the antidiabetic activity of aqueous extract of cinnamomum cassia in alloxan induced diabetic albino rats. Antidiabetic activity of aqueous extract of cinnamomum. Diabetes was induced by intraperitoneal injection of 120 mgkg b. Fresh liver weight and its relationship with body weight were. Alloxan monohydrate was used to induce diabetes in the rats. Hypoglycemic activity of cucumis sativus extract on alloxan. Antidiabetic effect of hydroalcoholic extract of carthamus. When the alcohol extract was fractionated by sequential solvent extraction, the activity was found in ethyl acetate fraction 50 mgkg. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Effects of aqueous extracts of lannea edulis in alloxaninduced diabetic rats. Effect of ethanolic extract of pterocarpus marsupium root. It is used as a cleansing and purifying agent and yet can be considered as a bodybuilder because of its nutritional value.
Hypoglycemic effects in alloxaninduced diabetic rats of the phenolic extract from mongolian oak cups enriched in ellagic acid, kaempferol. The effect of orally administered aqueous extracts 400 mgkg body weight of clitoria ternatea leaves and flowers on serum glucose, glycosylated hemoglobin, and insulin were examined in control and extracttreated diabetic rats. This study was therefore designed to establish the relative potency of insulin and r. Dose optimization of alloxan for diabetes in albino mice. Effect of zingiber officinale on some hematologic values.
After an overnight fast, the rats were injected single dose 125mgkg of freshly prepared 5% solution of alloxan monohydrate in 0. The mechanisms of alloxan and streptozotocininduced diabetes. Rats treated with 100 mgkg bw of methanol leaf extract of hibiscus sabdariffa also exhibited hibiscus sabdariffa. Diabetes was induced in male wistar rats by the administration of alloxan monohydrate at 100 mgkg of body weight. The prime focus of this study is to estimate the potential of ipomoea batatas l peeloff on magnesium and calcium level in alloxan induced diabetic rats. Partial recovery from alloxaninduced diabetes by sodium. The present study was carried out to evaluate the antidiabetic effect of psidium guajava leaves extract and to study the activities of hexokinase, aldolase and phosphor glucoisomerase, and gluconeogenic enzymes such asglucose6phosphatase and. Hypoglycemic effect of ginger zingiber officinale in alloxan. Oral administration of polyherbal formulation to diabetic animals up to four weeks dose dependently reduced the blood glucose level, which was comparable to that of glibenclamide 5 mgkg.
The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxan induced diabetic rats. Indeed, the essential oil of rosescented geranium leaves was prepared and their antioxidant activities as well as their hypoglycemic effects were studied. Hypoglycemic activity of cucumis sativus extract on. The effect of lisinopril on blood glucose level given alone. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. View the article pdf and any associated supplements and figures for. Blood glucose lowering activity of aloe based composition. The ethanol extracts of pterocarpus marsupium root was investigated for its antidiabetic effect in wistar albino rats. Experimental results also reflect that the tectona grandis is capable of reducing the oxidative state associated with diabetes 25. Marked degenerations of the islets of langer hans was observed in pancreas of alloxaninduced diabetic rats, whereas, histological examination.
Comparative effects of rothmannia hispida leaves extract and. The animals were distributed into 4 groups of 5 rats each namely. Antioxidant and antidiabetic activities of terminalia. Hypoglycemic effect of leucas lavandulaefolia willd in. Original article rat model of foodinduced nonobesetype 2. Rats treated with 7% protein isolate recorded the most significant p diabetic and hypoglycaemic effects on alloxan induced diabetic rats. The alloxan induced diabetic rats were grouped into three groups containing at least 5 rats in each group. This fraction containing steroids and coumarins showed antidiabetes activity in alloxan diabetic rats as judged from serum glucose levels, liver glycogen content and body weight. Hypoglycaemic activity and nephroprotectective effect of.
In the light of the above data, the objectives of the present study were to evaluate the effect of hcq on blood glucose and lipid profile in alloxan induced diabetic rats and to study its effect on the above parameters in com bination with conventional oral antidiabetic agents. One alternative therapeutic approach in dm patients is traditional use of herbal medicines such as physalis angulata herbs. Alloxan has been administered in single or multiple doses, through different routes. Furthermore, alloxan inhibits glucokinase, a shcontaining protein essential for insulin secretion induced by glucose. Streptozotocin use in mice and rats version 1 date of publication. In the experiment, six rats were used in each group. Diabetes induced by coxsackie virus infection is a direct. Antidiabetic drugs in combination with hydroxychloroquine. In alloxan induced diabetic rats by oral administration of extract 100mgkg body weight for 15 days. Antihyperglycemic and antihyperlipidemic effects of. In the in vivo experiment, glucose given to rats 20 min prior to alloxan partially restricted alloxan induced increase in the activity of glutathione peroxidase.
Pdf antidiabetic activity of extract on alloxan induced diabetic rats. Effects of aqueous avocado pear persea americana seed. This experimental study examined the impact of sildenafil citrate, vitamin a vit a, vitamin c vit c and vitamin e vit c on wound healing in a rat model of diabetes n100. Analysis of certain biochemical indices on alloxan induced. Antidiabetic activity evaluation of onobrychis species on alloxan. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Antioxidant, antihyperglycemic, and antihyperlipidemic. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Hypoglycemic effect of polyherbal formulation in alloxan. What is the right dose of alloxan to induce diabetes 1 in.
Diabetes was induced in albino rats by administration of alloxan monohydrate 100mgkg,i. Original article renoprotective effect of grape seed extract. In this study, diabetic mice were given 10 mgkg and 500 mgkg of glibenclamide and aloe, respectively, twice per day for 14 days. The experimental animals were grouped into five groups ae of 10 animals each. Alloxan diabetes was induced into 20 rats and the rats weighed 125g. The effects of dosage and the routes of administrations of. Effect of dme on alloxan induced diabetic rats a total of twenty five 25 diabetic and five 5 normoglycemic rats of both sexes were used. E, and ceruloplasmin in streptozotocininduced diabetic rats. Fresh leaves of the plant were collected from their local habitat in the university of nigeria, nsukka environment. The most plausible explanation for induction of diabetes due to alloxan. Anion gap toxicity in alloxan induced type 2 diabetic rats. Alloxaninduced diabetes triggers the development of.
Alloxan is a urea derivative which causes selective necrosis of the. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. The induction of diabetes was confirmed after the 5th day of alloxan treatment by. Apocynaceae leaf extract in alloxan induced diabetic albino rats. Hypoglycemic effects in alloxaninduced diabetic rats of the. Rothmannia hispida herb extract is also used to manage dm by traditional healers. There was significant increase in pcv, hb, rbc and wbc in diabetic rats.
The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. Jun 26, 2012 hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. An alloxaninduced diabetic mice test model was used. Diabetes in rodents is induced by injecting alloxan or streptozotocin which induce diabetes while producing ros leading to demolition of. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Hypoglycemic and antioxidant effects of leaf essential oil of. In this work, it was investigated the effects of diabetogenic doses of alloxan range from 80 to 140 mgkg 80,100,120, and 140 as a single dose by the routes of sc and ip administration. The treatment of alloxan induced diabetic rats with the tgm extract and significantly p diabetic group.
Antidiabetic activity of aqueous extract of sigesbeckia orientalis st. Antihyperglycemic and antihyperlipidemic activity of. Tirupathi mallareddy college of pharmacy, dundigal, rangareddy, andhra pradesh, india. Research article antidiabetic effect of sida cordata in alloxan induced diabetic rats naseeralishahandmuhammadrashidkhan department of biochemistry, faculty of. Rats with blood glucose levels above 250 mgdl were considered diabetic 30 and selected for the study. To establish its utility in diabetes mellitus, the present study evaluated the antidiabetic and antioxidant effects of cs in alloxan.
Alloxaninduced diabetes, a common model for evaluating. Anti diabetic and hypolipidaemic properties of ginger zingiber officinale in streptozotocin induced diabetic rats volume 96 issue 4 zainab m. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Please see the supplementary file for standards hplc chromatograms figure s1. Alloxan induced diabetic untreated rats showed a progressive decrease body weight figure 2 with final body weight loss of 32. The aqueous extract of cinnamon 60mgkg was studied alone and in combination with conventional oral antidiabetic drugs like glibenclamide 5mgkg and metformin 0.
Antihyperglycemic effect of gasca d herbal formulation on alloxan. In research it is used for induction of type 1 diabetes. Diabetes was induced by interperitoneal injection of alloxan monohydrate 150. Antidiabetic activity of leaves of jatropha curcus l. The oral administration of 200, 300, and 400mgkg b. Hypoglycemic effect of ginger zingiber officinale in alloxan induced diabetic rats rattus norvagicus 2011. Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan induced diabetic rats. Diabetes was induced in rats two weeks before starting the experiment. Twenty four albino rats were randomly allocated into four groups ad of six rats each such that group a diabetes control received 0.
It was observed that in normal rats the lisinopril slightly reduced the blood glucose level at all time points which was significant only at 30 and 120 min time points p. Research article alloxan induced diabetes mellitus in. Pdf antidiabetic activity of hf on alloxan induced diabetic rats. After 48 h, rats with fasting blood glucose levels of 200 mgdl and above were considered diabetic and used for the study. Dose optimization of alloxan for diabetes in albin. Alloxan injection produced severe diabetic renal damage, as revealed by significantly greater malondialdehyde mda levels and superoxide dismutase sod activity in the. Antidiabetic and antioxidant activities of the methanol leaf. Induction of diabetes mellitus alloxan monohydrate was used to induce diabetes mellitus. The mechanism of alloxan and streptozotocin action in b. In the alloxan induced diabetic rats showed that extract at 500 mgkg decreased significantly fasting plasma glucose level by 61. Pdf diabetes mellitus is affecting many in workforce, thus have a major impact on both individual and national productivity. The rats are divided into 6 groups and each group con tains 6 rats n 6 as follows.